• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
  • Amino Acid Range: Ser22-Asp182
Catalog #CD8-HM18A-UL
Price
Regular price $41745
Regular price Sale price $41745
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Size
Avantor VWR Fisher Scientific

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Human CD8 alpha/CD8A Protein (active dimer), Ultra Low Endotoxin (CD8-HM18A-UL) Summary

Product Description

 Recombinant Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser22-Asp182.

Product Background

 CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.

Human CD8 alpha/CD8A Protein (active dimer), Ultra Low Endotoxin (CD8-HM18A-UL) Specifications/Details

Protein
 CD8 alpha/CD8A
Synonyms
 CD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen;Lyt2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser22-Asp182
Molecular Weight
 The protein has a predicted MW of 18.69 kDa. Due to glycosylation, the protein migrates to 28-32 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. Immobilized Human CD8 alpha, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 alpha Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA.
Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD8 alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD8 alpha is greater than 95% and the molecular weight of this protein is around 40-50 kDa as determined by SEC-MALS. The purity of Human CD8 alpha is greater than 95% and the molecular weight of this protein is around 40-50 kDa as determined by SEC-MALS.
The purity of Human CD8 alpha is greater than 95% as determined by SEC-HPLC. The purity of Human CD8 alpha is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...