• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD8 beta, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
  • Amino Acid Range: Leu22-Pro170
Catalog #CD8-HM28B
Price
Regular price $345.00
Regular price Sale price $345.00
/
Size
Avantor VWR Fisher Scientific

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Human CD8 beta/CD8B Protein (CD8-HM28B) Summary

Product Description

 Recombinant Human CD8 beta/CD8B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Leu22-Pro170.

Product Background

 CD8 on thymocytes and thymus-derived T cells consists of disulfide-linked CD8a and CD8b chains, which are transmembrane proteins, containing N-terminal Ig domains, extended and glycosylated hinge and stalk regions, and transmembrane and cytoplasmic portions. The cytoplasmic portion of CD8β, mainly due to its palmitoylation, mediates partitioning of CD8 in lipid rafts, where it efficiently associates with p56 lck. In addition, the cytoplasmic portion of CD8β mediates constitutive association of CD8 with TCR/CD3. The resulting TCR-CD8 adducts exhibit high affinity for major histocom-patibility complex (MHC)-peptide.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CD8 beta/CD8B Protein (CD8-HM28B) Specifications/Details

Protein
 CD8 beta/CD8B
Synonyms
 CD8 alphaβ CD8A&CD8B;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu22-Pro170
Molecular Weight
 The protein has a predicted MW of 42.72 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD8 beta, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CD8 beta, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. Immobilized Human CD8 beta, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD8 beta Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Human CD8 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD8 beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD8 beta is greater than 95% as determined by SEC-HPLC. The purity of Human CD8 beta is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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