Product Description |
Recombinant Human CD96/TACTILE (C110S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Met503(C110S). |
Product Background |
The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development. |
Product Category |
Recombinant Protein |
Target |
CD96/TACTILE (C110S) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val22-Met503(C110S) |
Molecular Weight |
The protein has a predicted MW of 54.63 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD96 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD96 (C110S), His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC test). The affinity constant of 0.289 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96 (C110S), His Tag with an affinity constant of 0.289 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Anti-CD96 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD96 (C110S), His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC test). |
|
|
Human CD96 (C110S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD96 (C110S) is greater than 95% as determined by SEC-HPLC. |
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