Regular price
$346.50
Order by 4pm ET for delivery on:
Human CD96/TACTILE (C110S) Protein (CD9-HM19S) Summary
Product Description |
Recombinant Human CD96/TACTILE (C110S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Met503(C110S). |
Product Background |
The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Human CD96/TACTILE (C110S) Protein (CD9-HM19S) Specifications/Details
Protein |
CD96/TACTILE |
Synonyms |
CD96 molecule; CD96; DKFZp667E2122; TACTILE |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val22-Met503(C110S) |
Molecular Weight |
The protein has a predicted MW of 54.63 kDa. Due to glycosylation, the protein migrates to 100-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CD96 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD96 (C110S), His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC test). The affinity constant of 0.289 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96 (C110S), His Tag with an affinity constant of 0.289 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Anti-CD96 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD96 (C110S), His Tag with the EC50 of 28.7ng/ml determined by ELISA (QC test). |
|
|
Human CD96 (C110S) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD96 (C110S) is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
