Human CD96/TACTILE Protein (CD9-HM196)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Anti-CD96 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 0.22μg/ml determined by ELISA. The affinity constant of 0.958 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val22-Met503

Catalog: CD9-HM196

$432.00
Size:

Order now get it on

Product Description Recombinant Human CD96/TACTILE Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Val22-Met503.
Product Category Recombinant Protein
Target CD96/TACTILE
Synonyms CD96 molecule; CD96; DKFZp667E2122; TACTILE
Gene/Protein ID P40200-2
Species Human
Exact Sequence Val22-Met503
Molecular Weight The protein has a predicted MW of 54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-CD96 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 0.22μg/ml determined by ELISA. The affinity constant of 0.958 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 μM as determined in SPR assay (Biacore T200).
Immobilized Anti-CD96 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 0.22μg/ml determined by ELISA.
The purity of Human CD96 is greater than 95% as determined by SEC-HPLC.
Human CD96 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in