Human CD96/TACTILE Protein (CD9-HM196)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Anti-CD96 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 28.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Val22-Met503

Catalog: CD9-HM196

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Product Description	Recombinant Human CD96/TACTILE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val22-Met503.
Product Background	The receptors CD96 and TIGIT are expressed on the surface of T and natural killer (NK) cells, and recent studies suggest both play important inhibitory roles in immune function. CD96 has been shown to modulate immune cell activity in mice, with Cd96-/- mice displaying hypersensitive NK-cell responses to immune challenge and significant tumor resistance. The counterbalance between the putative inhibitory CD96 and TIGIT receptors and the activating receptor, CD226, offers unique strategies for immuno-oncology drug development.
Product Category	Recombinant Protein

Target	CD96/TACTILE
Synonyms	CD96 molecule; CD96; DKFZp667E2122; TACTILE
Gene/Protein ID	P40200-2
Species	Human
Exact Sequence	Val22-Met503
Molecular Weight	The protein has a predicted MW of 54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Anti-CD96 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 28.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.958 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 uM as determined in SPR assay (Biacore T200).
	Immobilized Anti-CD96 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD96, His Tag with the EC50 of 28.8ng/ml determined by ELISA (QC Test).
	The purity of Human CD96 is greater than 95% as determined by SEC-HPLC.
	Human CD96 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.