Name: Human CDCP1 Protein (CDC-HM101)
Brand: KactusBio
SKU: CDC-HM101-100UG
Price: 360.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe30-Leu666

Catalog #CDC-HM101

Size

100UG

500UG

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Product Description	Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666.
Product Background	Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Product Category	Recombinant Protein / Antibody discovery

Protein	CDCP1
Synonyms	CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession	Q9H5V8-1
Species	Human
Biotinylated	no
Amino Acid Range	Phe30-Leu666
Molecular Weight	The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200).
	Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
	Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC.