Product Description |
Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666. |
Product Background |
Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression. |
Product Category |
Recombinant Protein |
Target |
CDCP1 |
Synonyms |
CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe30-Leu666 |
Molecular Weight |
The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200). |
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|
Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). |
|
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Human CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC. |
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