Human CDCP1 Protein (CDC-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Phe30-Leu666

Catalog: CDC-HM101

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Product Description	Recombinant Human CDCP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe30-Leu666.
Product Background	Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.
Product Category	Recombinant Protein

Target	CDCP1
Synonyms	CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Gene/Protein ID	Q9H5V8-1
Species	Human
Exact Sequence	Phe30-Leu666
Molecular Weight	The protein has a predicted MW of 72.8 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.18nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CDCP1, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200).
	Immobilized Human CDCP1 at 2ug/ml (100ul/Well). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	The purity of Human CDCP1 is greater than 95% as determined by SEC-HPLC.
	Human CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.