Regular price
$375.00
Order by 4pm ET for delivery on:
Human CDH6/Cadherin 6 (EC4del) Protein (CDH-HM14D) Summary
Product Description |
Recombinant Human CDH6/Cadherin 6 (EC4del) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr19-Phe383 and Glu487-Ala615. |
Product Background |
Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human CDH6/Cadherin 6 (EC4del) Protein (CDH-HM14D) Specifications/Details
Protein |
CDH6/Cadherin 6 |
Synonyms |
Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr19-Phe383 & Glu487-Ala615 |
Molecular Weight |
The protein has a predicted MW of 56.46 kDa. Due to glycosylation, the protein migrates to 65-85 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CDH6 Antibody 2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6 (EC4del), His Tag with the EC50 of 0.25ug/ml determined by ELISA. The affinity constant of 0.96 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4). |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human CDH6 (EC4del), His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CDH6 Antibody 1, hFc Tag with an affinity constant of 0.96 uM as determined in SPR assay (Biacore S200). |
|
|
Immobilized Anti-CDH6 Antibody 2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6 (EC4del), His Tag with the EC50 of 0.25ug/ml determined by ELISA. |
|
|
Human CDH6 (EC4del) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CDH6 (EC4del) is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
