Human CDH6/Cadherin-6 Protein (CHD-HM106)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Activity: Immobilized Human CDH6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 85.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Thr19-Ala615

Catalog: CHD-HM106

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Product Description	Recombinant Human CDH6/Cadherin-6 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Thr19-Ala615.
Product Background	Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity.
Product Category	Recombinant Protein

Target	CDH6/Cadherin-6
Accession	P55285-1
Species	Human
Amino Acid Range	Thr19-Ala615
Molecular Weight	The protein has a predicted MW of 67.48 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CDH6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 85.1 ng/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CDH6, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CDH6 Antibody with an affinity constant of 1.41 nM as determined in SPR assay (Biacore T200).
	Immobilized Human CDH6, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CDH6 Antibody, Rabbit IgG Tag with the EC50 of 85.1 ng/ml determined by ELISA (QC Test).
	The purity of Human CDH6 is greater than 95% as determined by SEC-HPLC.
	Human CDH6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.