Human CDH6/Cadherin 6 Protein (CHD-HM106)
Human CDH6/Cadherin 6 Protein (CHD-HM106)
Human CDH6/Cadherin 6 Protein (CHD-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Thr19-Ala615
Catalog #CHD-HM106
Regular price $203.00
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Product Description

Recombinant Human CDH6/Cadherin 6 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Thr19-Ala615.

Product Background

Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity.

Product Category

Recombinant Protein / Antibody discovery

Protein

CDH6/Cadherin 6

Synonyms

Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr19-Ala615

Molecular Weight

The protein has a predicted MW of 67.48 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human CDH6, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CDH6 Antibody with an affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). Human CDH6, His Tag captured on CM5 Chip via Anti-His Antibody can bind Anti-CDH6 Antibody with an affinity constant of 1.41 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test). Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test).
Human CDH6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CDH6 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CDH6 is greater than 95% as determined by SEC-HPLC. The purity of Human CDH6 is greater than 95% as determined by SEC-HPLC.

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