- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
- Activity: Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Thr19-Ala615
Product Description |
Recombinant Human CDH6/Cadherin 6 Protein is expressed from HEK293 with His tag at the C-Terminus; It contains Thr19-Ala615. |
Product Background |
Cadherin 6 (CDH6) is an adhesion molecule localizing to the endometrial luminal epithelial cell surface in the mid-secretory/receptive phase and knockdown of CDH6 in the Ishikawa cells (receptive endometrial epithelial cell line) compromises cell integrity. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
CDH6/Cadherin 6 |
Synonyms |
Cadherin-6; Cadherin6; K-cadherin; CDH6; CAD6; KCAD; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr19-Ala615 |
Molecular Weight |
The protein has a predicted MW of 67.48 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-CDH6 antibody, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CDH6, His Tag with the EC50 of 1.85ug/ml determined by ELISA (QC Test). The affinity constant of 1.41 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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