Name: Human CEACAM-1/CD66a Protein (CEA-HM201)
Brand: KactusBio
SKU: CEA-HM201-100UG
Price: 223.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln35-Gly428

Catalog #CEA-HM201

Size

100UG

500UG

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Product Description	Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gly428.
Product Background	Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CEACAM-1/CD66a
Synonyms	BGP; BGP1; BGPI; CEACAM1; CD66a
Accession	P13688-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln35-Gly428
Molecular Weight	The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	Human CEACAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC.