ELISA binding curve for human CEACAM-1 (His tag) demonstrating OD450 absorbance in response to increasing concentrations of anti-CEACAM1 antibody (hFc tag).
Human CEACAM-1/CD66a Protein (CEA-HM201)
Human CEACAM-1/CD66a Protein (CEA-HM201)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln35-Gly428
Catalog #CEA-HM201
Regular price $223.30
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Product Description

Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gly428.

Product Background

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CEACAM-1/CD66a

Synonyms

BGP; BGP1; BGPI; CEACAM1; CD66a

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln35-Gly428

Molecular Weight

The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
Human CEACAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CEACAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC. The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC.

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