Name: Human CEACAM-1/CD66a Protein (CEA-HM201)
Brand: KactusBio
SKU: CEA-HM201-100UG
Price: 223.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln35-Gly428

Catalog #CEA-HM201

Size

100UG

500UG

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Product Description	Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gly428.
Product Background	Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	CEACAM-1/CD66a
Synonyms	BGP; BGP1; BGPI; CEACAM1; CD66a
Accession	P13688-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln35-Gly428
Molecular Weight	The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	Human CEACAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC.