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Human CEACAM-1/CD66a Protein, Ultra Low Endotoxin (CEA-HM201-UL) Summary
Product Description |
Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gly428. |
Product Background |
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Human CEACAM-1/CD66a Protein, Ultra Low Endotoxin (CEA-HM201-UL) Specifications/Details
Protein |
CEACAM-1/CD66a |
Synonyms |
BGP; BGP1; BGPI; CEACAM1; CD66a |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln35-Gly428 |
Molecular Weight |
The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). |
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Human CEACAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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