Human CEACAM-3 Protein (CEA-HM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CEACAM-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys35-Gly155
Catalog #CEA-HM103
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Product Description
 Recombinant Human CEACAM-3 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys35-Gly155.
Product Background
 The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.
Product Category
 Recombinant Protein
Target
 CEACAM-3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys35-Gly155
Molecular Weight
 The protein has a predicted MW of 14.19 kDa. Due to glycosylation, the protein migrates to 17-27 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CEACAM-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CEACAM-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 26.5ng/ml determined by ELISA.
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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