ELISA binding curve for human FGFR2 beta (IIIb) protein (His tag), showing OD450 absorbance vs. log anti-FGFR2 IIIb antibody concentration
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CEACAM-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA.
  • Amino Acid Range: Lys35-Gly155
Catalog #CEA-HM103
Regular price $297.00
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Size
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Human CEACAM-3/CD66d Protein (CEA-HM103) Summary

Product Description

Recombinant Human CEACAM-3/CD66d Protein is expressed from HEK293 with His tag at the C-terminus.It contains Lys35-Gly155.

Product Background

The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Human CEACAM-3/CD66d Protein (CEA-HM103) Specifications/Details

Protein
CEACAM-3/CD66d
Synonyms
CEACAM3; CD66d; CGM1; CEA;
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Lys35-Gly155
Molecular Weight
The protein has a predicted MW of 14.19 kDa. Due to glycosylation, the protein migrates to 17-27 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CEACAM-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CEACAM-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA. Immobilized Human CEACAM-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-7, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA.
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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