Human CEACAM-3/CD66d Protein (CEA-HM203)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys35-Gly155
Catalog #CEA-HM203
Regular price $270.00
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Product Description

Recombinant Human CEACAM-3/CD66d Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Lys35-Gly155.

Product Background

The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.

Product Category

Recombinant Protein

Protein

CEACAM-3/CD66d

Synonyms

CEACAM3; CD66d; CGM1; CEA;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Lys35-Gly155

Molecular Weight

The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC. The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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