Product Description |
Recombinant Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Lys35-Gly155. |
Product Background |
The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac. |
Product Category |
Recombinant Protein |
Target |
CEACAM-3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys35-Gly155 |
Molecular Weight |
The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. |
|
|
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC. |
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