• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-7, His Tag (Cat. CEA-HM107) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 28.7ng/ml determined by ELISA.
  • Amino Acid Range: Lys35-Gly155
Catalog #CEA-HM203
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human CEACAM-3/CD66d Protein (CEA-HM203) Summary

Product Description

 Recombinant Human CEACAM-3/CD66d Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Lys35-Gly155.

Product Background

 The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets

Human CEACAM-3/CD66d Protein (CEA-HM203) Specifications/Details

Protein
 CEACAM-3/CD66d
Synonyms
 CEACAM3; CD66d; CGM1; CEA;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys35-Gly155
Molecular Weight
 The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CEACAM-7, His Tag (Cat. CEA-HM107) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 28.7ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CEACAM-7, His Tag (Cat. CEA-HM107) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 28.7ng/ml determined by ELISA. Immobilized Human CEACAM-7, His Tag (Cat. CEA-HM107) at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 28.7ng/ml determined by ELISA.
Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CEACAM-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC. The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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