Human CEACAM-3 Protein (CEA-HM203)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Lys35-Gly155

Catalog: CEA-HM203

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Product Description	Recombinant Human CEACAM-3 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Lys35-Gly155.
Product Background	The human granulocyte-specific receptor carcinoembryonic antigen-related cell adhesion molecule (CEACAM)3 is critically involved in the opsonin-independent recognition of several bacterial pathogens. CEACAM3-mediated phagocytosis depends on the integrity of an ITAM-like sequence within the cytoplasmic domain of CEACAM3 and is characterized by rapid stimulation of the GTPase Rac.
Product Category	Recombinant Protein

Target	CEACAM-3
Gene/Protein ID	P40198-1
Species	Human
Exact Sequence	Lys35-Gly155
Molecular Weight	The protein has a predicted MW of 39.85 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA.
	The purity of Human CEACAM-3 is greater than 95% as determined by SEC-HPLC.
	Human CEACAM-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.