Name: Human CEACAM-5/CD66e (501-685) Protein, Ultra Low Endotoxin (CAM-HM1D3-UL)
Brand: KactusBio
SKU: CAM-HM1D3-UL-100UG
Price: 417.0 USD
Availability: InStock

Human CEACAM-5/CD66e (501-685) Protein, Ultra Low Endotoxin (CAM-HM1D3-UL)

Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).

Human CEACAM-5 (501-685) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CEACAM-5 (501-685) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Amino Acid Range: Pro501-Ala685

Catalog #CAM-HM1D3-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human CEACAM-5/CD66e (501-685) Protein, Ultra Low Endotoxin (CAM-HM1D3-UL) Summary

Product Description	Recombinant Human CEACAM-5/CD66e (501-685) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro501-Ala685.
Product Background	Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Human CEACAM-5/CD66e (501-685) Protein, Ultra Low Endotoxin (CAM-HM1D3-UL) Specifications/Details

Protein	CEACAM-5/CD66e
Synonyms	CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392
Accession	P06731-1
Species	Human
Biotinylated	no
Amino Acid Range	Pro501-Ala685
Molecular Weight	The protein has a predicted MW of 20.89 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.001 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA (QC Test).
	Human CEACAM-5 (501-685) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CEACAM-5 (501-685) is greater than 95% as determined by SEC-HPLC.