Human CEACAM-7 Protein (CEA-HM107)

Human CEACAM-1, His Tag (Cat. CEA-HM201) immobilized on CM5 Chip can bind Human CEACAM-7, His Tag- with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).

Immobilized Human CEACAM-7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag (Cat. CEA-HM203) with the EC50 of 28.7ng/ml determined by ELISA.

Human CEACAM-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CEACAM-7 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag (Cat. CEA-HM203) with the EC50 of 28.7ng/ml determined by ELISA. The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Thr36-Ser242

Catalog #CEA-HM107

Price

$297⁰⁰

Size

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Human CEACAM-7 Protein (CEA-HM107) Summary

Product Description

Recombinant Human CEACAM-7 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr36-Ser242.

Product Background

Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to a group of mammalian immunoglobulin-related glycoproteins. They are involved in cell–cell recognition and modulate cellular processes that range from the shaping of tissue architecture and neovascularization to the regulation of insulin homeostasis and T-cell proliferation. CEACAM7 (CGM2), a member of the CEA family of proteins with expression restricted to the colon and pancreas, as a potential CAR T-cell target for PDAC.

Human CEACAM-7 Protein (CEA-HM107) Specifications/Details

Protein	CEACAM-7
Synonyms	CGM2; CEACAM7; CEA
Accession	Q14002-1
Species	Human
Biotinylated	no
Amino Acid Range	Thr36-Ser242
Molecular Weight	The protein has a predicted MW of 24.45 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CEACAM-7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag (Cat. CEA-HM203) with the EC50 of 28.7ng/ml determined by ELISA. The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human CEACAM-1, His Tag (Cat. CEA-HM201) immobilized on CM5 Chip can bind Human CEACAM-7, His Tag- with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CEACAM-7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag (Cat. CEA-HM203) with the EC50 of 28.7ng/ml determined by ELISA.
	Human CEACAM-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CEACAM-7 is greater than 95% as determined by SEC-HPLC.