Human CEACAM-7 Protein (CEA-HM107)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Thr36-Ser242

Catalog: CEA-HM107

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Product Description	Recombinant Human CEACAM-7 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr36-Ser242.
Product Background	Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to a group of mammalian immunoglobulin-related glycoproteins. They are involved in cell–cell recognition and modulate cellular processes that range from the shaping of tissue architecture and neovascularization to the regulation of insulin homeostasis and T-cell proliferation. CEACAM7 (CGM2), a member of the CEA family of proteins with expression restricted to the colon and pancreas, as a potential CAR T-cell target for PDAC.
Product Category	Recombinant Protein

Target	CEACAM-7
Gene/Protein ID	Q14002-1
Species	Human
Exact Sequence	Thr36-Ser242
Molecular Weight	The protein has a predicted MW of 24.45 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag- with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CEACAM-7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CEACAM-3, hFc Tag with the EC50 of 31.1ng/ml determined by ELISA (QC Test).
	The purity of Human CEACAM-7 is greater than 95% as determined by SEC-HPLC.
	Human CEACAM-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.