Human CEACAM-8/CD66b Protein (CEA-HM108)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 4.59 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln35-Ser319
Catalog #CEA-HM108
Regular price $240.00
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Product Description
Recombinant Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Ser319.
Product Background
Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.
Product Category
Recombinant Protein
Target
CEACAM-8/CD66b
Synonyms
CD66b; CEACAM8; CGM6; CD67; NCA-95
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln35-Ser319
Molecular Weight
The protein has a predicted MW of 32.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 4.59 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 4.59 uM as determined in SPR assay (Biacore T200).
Human CEACAM-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.

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