Human CHRDL1 Protein (CDL-HE101)
Human CHRDL1 Protein (CDL-HE101)
Human CHRDL1 Protein (CDL-HE101)
Human CHRDL1 Protein (CDL-HE101)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CHRDL1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu28-Cys456
Catalog #CDL-HE101
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Product Description
 Recombinant Human CHRDL1 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Glu28-Cys456.
Product Background
 CHRDL1 (Chordin-like 1) is a secreted protein that acts as an antagonist of bone morphogenetic protein (BMP). BMP plays a role as an activator of BMP receptor II (BMPR II), which mediates extracellular to intracellular signal transmission and is involved in carcinogenesis and metastasis.The hypermethylation of the CHRDL1 promoter in gastric cancer, which induced low expression of CHRDL1 and decreased its secretion to the supernatant.
Product Category
 Recombinant Protein
Target
 CHRDL1
Synonyms
 Neuralin-1; Neurogenesin-1; Ventroptin; CHRDL1; NRLN1; CHL; Chordin-like 1; CRDL1; dA141H5.1; VOPT
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu28-Cys456
Molecular Weight
 The protein has a predicted MW of 50 kDa same as Bis-Tris PAGE result.
Activity
 Immobilized Human CHRDL1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 E.coli
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CHRDL1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CHRDL1 Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
Human CHRDL1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
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