ELISA binding curve for Human CLEC-1, hFc Tag. The graph plots mean absorbance (OD450) against the logarithmic concentration of biotinylated anti-CLEC-1 antibody, showing a sigmoidal curve indicative of specific binding.
Human CLEC-1 Protein (CLE-HM201)
ELISA binding curve for Human CLEC-1, hFc Tag. The graph plots mean absorbance (OD450) against the logarithmic concentration of biotinylated anti-CLEC-1 antibody, showing a sigmoidal curve indicative of specific binding.
Human CLEC-1 Protein (CLE-HM201)
  • Express System: HEK293
  • Product Tag: N-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CLEC-1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA.
  • Amino Acid Range: Gln74-Asp280
Catalog #CLE-HM201
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Size
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Human CLEC-1 Protein (CLE-HM201) Summary

Product Description

 Recombinant Human CLEC-1 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Gln74-Asp280.

Product Background

 C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1β production in response to A. fumigatus keratitis.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human CLEC-1 Protein (CLE-HM201) Specifications/Details

Protein
 CLEC-1
Synonyms
 CLEC-1; CLEC1; CLEC1A; MGC34328
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln74-Asp280
Molecular Weight
 The protein has a predicted MW of 51.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CLEC-1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA.
Product Tag
 N-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CLEC-1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA. Immobilized Human CLEC-1, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC-1 Antibody, hFc Tag with the EC50 8.8ng/ml determined by ELISA.
Human CLEC-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CLEC-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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