Name: Human CLEC9A Protein (CLE-HM29A)
Brand: KactusBio
SKU: CLE-HM29A-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human CLEC9A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Amino Acid Range: Lys57-Val241
Other Available Proteins:

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Catalog #CLE-HM29A

Size

100UG

500UG

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Human CLEC9A Protein (CLE-HM29A) Summary

Product Description	Recombinant Human CLEC9A Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Lys57-Val241.
Product Background	CLEC9A is a recently discovered C-type lectin receptor involved in sensing necrotic cells. In humans, this receptor is selectively expressed by BDCA3(+) myeloid dendritic cells (mDCs), which have been proposed to be the main human cross-presenting mDCs and may represent the human homologue of murine CD8(+) DCs. In mice, it was demonstrated that antigens delivered with antibodies to CLEC9A are presented by CD8(+) DCs to both CD4(+) and CD8(+) T cells and induce antitumor immunity in a melanoma model.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CLEC9A Protein (CLE-HM29A) Specifications/Details

Protein	CLEC9A
Synonyms	CD370; CLEC9A; 9830005G06Rik; DNGR-1; HEEE9341; UNQ9341
Accession	Q6UXN8
Species	Human
Biotinylated	no
Amino Acid Range	Lys57-Val241
Molecular Weight	The protein has a predicted MW of 48.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CLEC9A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Product Tag	N-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CLEC9A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CLEC9A Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
	Human CLEC9A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CLEC9A is greater than 90% as determined by SEC-HPLC.