Human Coagulation factor III/Tissue Factor Protein, Ultra Low Endotoxin (TFR-HM203-UL)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Coagulation Factor III, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser33-Glu251
Catalog #TFR-HM203-UL
Regular price $281.00
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Product Description

Recombinant Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Ser33-Glu251.

Product Background

Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Coagulation factor III/Tissue Factor

Synonyms

Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ser33-Glu251

Molecular Weight

The protein has a predicted MW of 50.74 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Coagulation Factor III, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 0.01 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Coagulation Factor III, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA. Immobilized Human Coagulation Factor III, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Tissue Factor Antibody, hFc Tag with the EC50 of 30.2ng/ml determined by ELISA.
Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Coagulation factor III is greater than 95% as determined by SEC-HPLC. The purity of Human Coagulation factor III is greater than 95% as determined by SEC-HPLC.

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