• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >200 pmol/min/µg.
  • Amino Acid Range: Asn32-Lys488
Catalog #CFX-HM201
Price
Regular price $315.00
Regular price Sale price $315.00
/
Size
Avantor VWR Fisher Scientific

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Human Coagulation factor X Protein (pro form) (CFX-HM201) Summary

Product Description

 Recombinant Human Coagulation factor X Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Asn32-Lys488.

Product Background

 Coagulation factor X (FX), originally named Stuart-Prower factor, plays a pivotal role in the coagulation cascade, activating thrombin to promote platelet plug formation and prevent excess blood loss. Genetic variants in F10 may lead to FX deficiency and to impaired coagulation. FX variants are phenotypically classified as being type I, with the concomitant reduction of FX coagulant activity and FX antigen levels or type II, corresponding to a reduction in activity with normal antigen plasma levels.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Coagulation factor X Protein (pro form) (CFX-HM201) Specifications/Details

Protein
 Coagulation factor X
Synonyms
 Coagulation factor X; FX; F10; Stuart factor;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn32-Lys488
Molecular Weight
 The protein has a predicted MW of 77.32 kDa. Due to the furin cleavage site, the protein is cleaved into light and heavy chains and migrates as 25 kDa, 80-90 kDa and 100-120 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >200 pmol/min/µg.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM HEPES, 150mM NaCl, 100mM L-Arginine (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Coagulation factor X on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Coagulation factor X on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Coagulation factor X is greater than 90% as determined by SEC-HPLC. The purity of Human Coagulation factor X is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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