Human Coagulation factor XI (C321S) Protein (pro form) (FXI-HM1CS)

Immobilized Human Coagulation Factor XI (C321S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.

Human Coagulation factor XI (C321S) on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

The purity of Human Coagulation factor XI (C321S) is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human Coagulation Factor XI (C321S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.
Amino Acid Range: Glu19-Val625(C321S)

Catalog #FXI-HM1CS

Price

$390⁰⁰

Size

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Human Coagulation factor XI (C321S) Protein (pro form) (FXI-HM1CS) Summary

Product Description

Recombinant Human Coagulation factor XI (C321S) Protein (pro form) is expressed from HEK293 with His tag at the C-terminus. The protein needs to be activated by Thermolysin for an activated form.It contains Glu19-Val625 (C321S).

Product Background

Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.

Human Coagulation factor XI (C321S) Protein (pro form) (FXI-HM1CS) Specifications/Details

Protein	Coagulation factor XI
Synonyms	Coagulation factor XI; FXI; PTA; F11;
Accession	P03951-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu19-Val625(C321S)
Molecular Weight	The protein has a predicted MW of 69.62 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Coagulation Factor XI (C321S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >100 pmoles/min/ug.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 25mM HEPES, 150mM NaCl (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Coagulation Factor XI (C321S), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-factor XI Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
	Human Coagulation factor XI (C321S) on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	The purity of Human Coagulation factor XI (C321S) is greater than 90% as determined by SEC-HPLC.