Product Description |
Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676. |
Product Background |
Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation; C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex. |
Product Category |
Recombinant Protein |
Target |
Complement C5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln19-Cys1676 |
Molecular Weight |
Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). |
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Human Complement C5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Complement C5 is greater than 95% as determined by SEC-HPLC. |
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