Human Complement C5 Protein (CC5-HM1C5)
Catalog: CC5-HM1C5
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Product Description | Recombinant Human Complement C5 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln19-Cys1676. |
Product Category | Recombinant Protein |
Target | Complement C5 |
Gene/Protein ID | P01031-1 |
Species | Human |
Exact Sequence | Gln19-Cys1676 |
Molecular Weight | Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result. |
Activity | Immobilized Human Complement C5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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