Human Complement C5 Protein (CC5-HM1C5)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Complement C5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln19-Cys1676

Catalog: CC5-HM1C5

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Product Description Recombinant Human Complement C5 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln19-Cys1676.
Product Category Recombinant Protein
Target Complement C5
Gene/Protein ID P01031-1
Species Human
Exact Sequence Gln19-Cys1676
Molecular Weight Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result.
Activity Immobilized Human Complement C5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Complement C5, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA.
The purity of Human Complement C5 is greater than 95% as determined by SEC-HPLC.
Human Complement C5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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