Human Complement C5 Protein (CC5-HM1C5)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Complement C5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln19-Cys1676

Catalog: CC5-HM1C5

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Product Description	Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676.
Product Background	Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation; C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex.
Product Category	Recombinant Protein

Target	Complement C5
Gene/Protein ID	P01031-1
Species	Human
Exact Sequence	Gln19-Cys1676
Molecular Weight	Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Tris-Bis PAGE result.
Activity	Immobilized Human Complement C5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Complement C5, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA.
	The purity of Human Complement C5 is greater than 95% as determined by SEC-HPLC.
	Human Complement C5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.