Name: Human Complement C5 Protein (CC5-HM1C5)
Brand: KactusBio
SKU: CC5-HM1C5-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gln19-Cys1676

Catalog #CC5-HM1C5

Size

100UG

500UG

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Product Description	Recombinant Human Complement C5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln19-Cys1676.
Product Background	Complement component 5 (C5) is an essential factor of the defensive complement system in all vertebrates.C5 plays a major role in complement activation; C5 convertase cleaves C5 into the pro-inflammatory C5a, and C5b, the nidus for the formation of the lytic membrane attack complex.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Complement C5
Synonyms	Complement C5; C5; CPAMD4; ECLZB; C5a; C5b; C5D
Accession	P01031-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln19-Cys1676
Molecular Weight	Human Complement C5 Protein is cleaved into α chain and β chain by the enzyme lysis. Each chain has a predicted MW of 112.5 kDa (α chain) and 73.29 (β chain). Due to glycosylation, the protein migrates to 110-114 kDa (α chain) and 69-73 (β chain) based on Bis-Tris PAGE result.
Activity	Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Complement C5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Complement C5 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA (QC Test).
	Human Complement C5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Complement C5 is greater than 95% as determined by SEC-HPLC.