Human Complement C1s Protein (C1S-HM101) Summary
Product Description |
Recombinant Human Complement C1s Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu16-Asp688. |
Product Background |
Complement C1s protease inhibitors have potential utility in the treatment of diseases associated with activation of the classical complement pathway such as humorally mediated graft rejection, ischemia-reperfusion injury (IRI), vascular leak syndrome, and acute respiratory distress syndrome (ARDS). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Complement C1s Protein (C1S-HM101) Specifications/Details
Protein |
Complement C1s |
Synonyms |
EDSPD2; C1 esterase;Complement Component C1s |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu16-Asp688 |
Molecular Weight |
The protein has a predicted MW of 75.98 kDa. Due to enzyme lysis glycosylation, the protein migrates to 55-60 kDa (light chain), 32-35 kDa (heavy chain) and 76-96 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Complement C1s, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-C1S Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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