Human Complement Factor D/CFD Protein (CFD-HM101)
Human Complement Factor D/CFD Protein (CFD-HM101)
Human Complement Factor D/CFD Protein (CFD-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ile26-Ala253
Catalog #CFD-HM101
Regular price $346.50
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Product Description

Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile26-Ala253.

Product Background

Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium.

Product Category

Recombinant Protein / Antibody discovery

Protein

Complement Factor D/CFD

Synonyms

Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ile26-Ala253

Molecular Weight

The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test).
Human Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC. The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC.

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