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Human Complement Factor D/CFD Protein, Ultra Low Endotoxin (CFD-HM101-UL) Summary
Product Description |
Recombinant Human Complement Factor D/CFD Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile26-Ala253. |
Product Background |
Complement factor D is a serine protease essential for the activation of the alternative pathway and is expressed in the kidney, adipocytes, and macrophages. Factor D is found at relatively high levels in glomeruli suggesting that this component of the complement cascade could influence renal pathophysiology.Complement factor D or alternative pathway activation is needed to prevent spontaneous accumulation of C3 and IgM deposits within the mesangium. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human Complement Factor D/CFD Protein, Ultra Low Endotoxin (CFD-HM101-UL) Specifications/Details
Protein |
Complement Factor D/CFD |
Synonyms |
Adipsin; C3 convertase activator; Complement factor D; CFD; PFD; DF; ADN; FACTOR D; AMBP-1; EC 3.4.21; EC 3.4.21.46 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ile26-Ala253 |
Molecular Weight |
The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 26-27 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmoles/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human Complement Factor D, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody, hFc Tag with the EC50 of 18.1ng/ml determined by ELISA (QC Test). |
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Human Complement Factor D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Complement Factor D is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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