Human CRTAM Protein (CRM-HM101)
Human CRTAM Protein (CRM-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CRTAM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser18-Gly287
Catalog #CRM-HM101
Regular price $270.00
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Product Description
Recombinant Human CRTAM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser18-Gly287.
Product Background
Class-I Restricted T Cell-Associated Molecule (CRTAM) is a protein that is expressed after T cell activation. The interaction of CRTAM with its ligand, nectin-like 2 (Necl2), is required for the efficient production of IL-17, IL-22, and IFNγ by murine CD4 T cells, and it plays a role in optimal CD8 T and NK cell cytotoxicity. CRTAM promotes the pro-inflammatory cytokine profile; therefore, it may take part in the immunopathology of autoimmune diseases such as diabetes type 1 or colitis.
Product Category
Recombinant Protein
Target
CRTAM
Synonyms
CD355 antigen; CD355; CRTAM
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser18-Gly287
Molecular Weight
The protein has a predicted MW of 31.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CRTAM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CRTAM, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 15.5ng/ml determined by ELISA.
Human CRTAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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