Human CTGF/CCN2 Protein (CGF-HM101)
Human CTGF/CCN2 Protein (CGF-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human CTGF, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.
  • Amino Acid Range: Gln27-Ala349
Catalog #CGF-HM101
Regular price $346.50
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Size
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Human CTGF/CCN2 Protein (CGF-HM101) Summary

Product Description

Recombinant Human CTGF/CCN2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Ala349.

Product Background

Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis. CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human CTGF/CCN2 Protein (CGF-HM101) Specifications/Details

Protein
CTGF/CCN2
Synonyms
IGFBP8; IBP-8; CCN2; NOV2; HCS24; CTGF; CTGRP; Fisp12; MGC102839
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln27-Ala349
Molecular Weight
The protein has a predicted MW of 36.56 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CTGF, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 200mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human CTGF, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA. Immobilized Human CTGF, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CTGF Antibody, hFc Tag with the EC50 of 6.7 ng/ml determined by ELISA.
Human CTGF on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human CTGF on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Human CTGF is greater than 90% as determined by SEC-HPLC. The purity of Human CTGF is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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