Human CTGF/CCN2 Protein (CGF-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CTGF, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln27-Ala349

Catalog: CGF-HM201

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Product Description	Recombinant Human CTGF/CCN2 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gln27-Ala349.
Product Background	Connective tissue growth factor (CTGF) is a member of the CCN matricellular protein family, consisting of four domains, that regulates the signaling of other growth factors and promotes kidney fibrosis.CTGF can simultaneously interact with several factors with its four domains. The microenvironment differs depending on the types of cells and tissues and differentiation stages of these cells.
Product Category	Recombinant Protein

Target	CTGF/CCN2
Gene/Protein ID	Q5M8T4
Species	Human
Exact Sequence	Gln27-Ala349
Molecular Weight	The protein has a predicted MW of 61.77 kDa. Due to glycosylation, the protein migrates to 50-55 kDa and 62-72 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CTGF, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CTGF, hFc Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CTGF Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
	The purity of Human CTGF is greater than 95% as determined by SEC-HPLC.
	Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.