Human CXCL13/BCA-1 Protein (CXC-HM213)
Human CXCL13/BCA-1 Protein (CXC-HM213)
Human CXCL13/BCA-1 Protein (CXC-HM213)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human CXCL13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val23-Per109
Catalog #CXC-HM213
Regular price $300.00
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Product Description
Recombinant Human CXCL13/BCA-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val23-Per109.
Product Background
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice.
Product Category
Recombinant Protein
Target
CXCL13/BCA-1
Synonyms
ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Val23-Per109
Molecular Weight
The protein has a predicted MW of 37.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CXCL13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-hFc
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CXCL13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test).
Human CXCL13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CXCL13/BCA-1 Protein is greater than 95% as determined by SEC-HPLC.

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