Product Description |
Recombinant Human CXCL13/BCA-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val23-Per109. |
Product Background |
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
CXCL13/BCA-1 |
Synonyms |
ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val23-Per109 |
Molecular Weight |
The protein has a predicted MW of 37.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CXCL13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human CXCL13, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 31.3ng/ml determined by ELISA (QC Test). |
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Human CXCL13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human CXCL13/BCA-1 Protein is greater than 95% as determined by SEC-HPLC. |
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