- Express System: HEK293
- Product Tag: N-hFc
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human CXCL13, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Val23-Per109
Product Description |
Recombinant Human CXCL13/BCA-1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Val23-Per109. |
Product Background |
Recent studies have implicated chemokines in microglial activation and pathogenesis of neuropathic pain. C-X-C motif chemokine 13 (CXCL13) is a B lymphocyte chemoattractant that activates CXCR5. Using the spinal nerve ligation (SNL) model of neuropathic pain, CXCL13 was persistently upregulated in spinal cord neurons after SNL, resulting in spinal astrocyte activation via CXCR5 in mice. |
Product Category |
Recombinant Protein |
Target |
CXCL13/BCA-1 |
Synonyms |
ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val23-Per109 |
Molecular Weight |
The protein has a predicted MW of 37.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CXCL13, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CXCL13 Antibody, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 50mM Tris, 500mM NaCl (pH 7.4). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Related Products
Recently viewed