Name: Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM228)
Brand: KactusBio
SKU: CD8-HM228-100UG
Price: 150.0 USD
Availability: InStock

Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM228)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 46.3ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asn19-Pro152

Catalog #CD8-HM228

Size

100UG

500UG

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Product Description	Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn19-Pro152.
Product Background	CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Category	Recombinant Protein

Target	CD28
Synonyms	CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Accession	P10747-1
Species	Human/Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Asn19-Pro152
Molecular Weight	The protein has a predicted MW of 42.6 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 46.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human/Cynomolgus/Rhesus macaque CD28, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD28 Antibody, hFc Tag with the EC50 of 46.3ng/ml determined by ELISA.
	Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.

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