Name: Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM328)
Brand: KactusBio
SKU: CD8-HM328-100UG
Price: 297.0 USD
Availability: InStock

Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM328)

Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 42.2ng/ml determined by ELISA.

Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human/Cynomolgus/Rhesus macaque CD28 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 42.2ng/ml determined by ELISA.
Amino Acid Range: Asn19-Pro152

Catalog #CD8-HM328

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM328) Summary

Product Description	Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-Terminus.It contains Asn19-Pro152.
Product Background	CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM328) Specifications/Details

Protein	CD28
Synonyms	CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44
Accession	P10747-1
Species	Human/Cynomolgus/Rhesus macaque
Biotinylated	no
Amino Acid Range	Asn19-Pro152
Molecular Weight	The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 42.2ng/ml determined by ELISA.
Product Tag	N-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 42.2ng/ml determined by ELISA.
	Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human/Cynomolgus/Rhesus macaque CD28 is greater than 95% as determined by SEC-HPLC.