Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM328)

  • Express System: HEK293
  • Product Tag: N-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asn19-Pro152

Catalog: CD8-HM328

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Product Description Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with mFc (IgG2a) tag at the N-terminal.It contains Asn19-Pro152.
Product Category Recombinant Protein
Target CD28
Synonyms CD28 antigen (Tp44); CD28 antigen; CD28 molecule; CD28; MGC138290; T-cell-specific surface glycoprotein CD28; Tp44
Gene/Protein ID P10747-1
Species Human/Cynomolgus/Rhesus macaque
Exact Sequence Asn19-Pro152
Molecular Weight The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA. See testing image for detail.
Product Tag N-mFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human/Cynomolgus/Rhesus macaque CD28, mFc (IgG2a) Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA.
Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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