Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test). The affinity constant of 5.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn19-Pro152
Catalog #CD8-HM428
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Product Description
 Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.
Product Background
 CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins. When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Product Category
 Recombinant Protein
Target
 CD28
Synonyms
 CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Accession
Species
 Human/Cynomolgus/Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Asn19-Pro152
Molecular Weight
 The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test). The affinity constant of 5.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 N-His-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15 nM as determined in a SPR assay (Biacore T200).
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 23.9 ng/ml determined by ELISA (QC Test).
Human/Cynomolgus/Rhesus macaque CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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