Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asn19-Pro152

Catalog: CD8-HM428

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Product Description Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Asn19-Pro152.
Product Category Recombinant Protein
Target CD28
Synonyms CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Gene/Protein ID P10747-1
Species Human/Cynomolgus/Rhesus macaque
Exact Sequence Asn19-Pro152
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.
The purity of Human/Cynomolgus/Rhesus macaque CD28 was greater than 95% as determined by SEC-HPLC.
Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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