Human/Cynomolgus/Rhesus macaque CD28 Protein (CD8-HM428)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn19-Pro152

Catalog: CD8-HM428

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Product Description Recombinant Human/Cynomolgus/Rhesus macaque CD28 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Asn19-Pro152.

Product Background

CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Product Category

Recombinant Protein

Target

CD28
Synonyms CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290
Accession

Species

Human/Cynomolgus/Rhesus macaque

Amino Acid Range

Asn19-Pro152
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 36-52 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). The affinity constant of 5.15nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CD28 antibody captured on Protein A chip, can bind Human/Cynomolgus/Rhesus macaque CD28, His Tag with an affinity constant of 5.15nM as determined in a SPR assay (Biacore T200).
Human B7-1, hFc Tag captured on CM5 Chip via Protein A can bind Human/Cynomolgus/Rhesus macaque CD28, His-Avi Tag with an affinity constant of 43.61 uM as determined in SPR assay (Biacore T200).
Immobilized Human/Cynomolgus/Rhesus macaque CD28, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD28 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test).
Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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