Human DcR1/TRAILR3 Protein (TRA-HM103)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Tyr24-Ala236

Catalog: TRA-HM103

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Product Description	Recombinant Human DcR1/TRAILR3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Tyr24-Ala236.
Product Background	The tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family, which mediates apoptosis by the extrinsic pathway. Up-regulation of decoy receptors, DcR1 and DcR2, may result in diminished binding of TRAIL to their functional receptors. DcR1 expression occurs in a subset of EC and may contribute to resistance to TRAIL-induced apoptosis.
Product Category	Recombinant Protein

Target	DcR1/TRAILR3
Synonyms	CD263; DCR1; DCR1-TNFR; LIT; TRAIL-R3; TRAILR3; TRID; TNFRSF10C
Gene/Protein ID	Q05D71
Species	Human
Exact Sequence	Tyr24-Ala236
Molecular Weight	The protein has a predicted MW of 23.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.57 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human DcR1, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay (Biacore T200).
	The purity of Human DcR1/TRAILR3 is greater than 95% as determined by SEC-HPLC.
	Human DcR1/TRAILR3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.