Product Description |
Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417. |
Product Background |
Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts. |
Product Category |
Recombinant Protein |
Target |
DDR1 |
Synonyms |
HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; CD167a; EC 2.7.10.1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp21-Ala417 |
Molecular Weight |
The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DDR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human DDR1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA (QC Test). |
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Human DDR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC. |
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