Human DDR1 Protein (DDR-HM1R1)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DDR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Asp21-Ala417

Catalog: DDR-HM1R1

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Product Description Recombinant Human DDR1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp21-Ala417.

Product Background

Discoidin domain receptor1 (DDR1) is a collagen activated receptor tyrosine kinase and an attractive anti-fibrotic target. Its expression is mainly limited to epithelial cells located in several organs including skin, kidney, liver and lung. DDR1's biology is elusive, with unknown downstream activation pathways; however, it may act as a mediator of the stromal-epithelial interaction, potentially controlling the activation state of the resident quiescent fibroblasts.

Product Category

Recombinant Protein

Target

DDR1
Synonyms HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; CD167a; EC 2.7.10.1

Gene/Protein ID

Q08345-1

Species

Human

Exact Sequence

Asp21-Ala417
Molecular Weight The protein has a predicted MW of 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human DDR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). The affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200).
Immobilized Human DDR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test).
The purity of Human DDR1 is greater than 95% as determined by SEC-HPLC.
Human DDR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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