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Human DKK1 C terminal Domain Protein (DKK-HM31C) Summary
Product Description |
Recombinant Human DKK1 C terminal Domain Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Met178-His266. |
Product Background |
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human DKK1 C terminal Domain Protein (DKK-HM31C) Specifications/Details
Protein |
DKK1 |
Synonyms |
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met178-His266 |
Molecular Weight |
The protein has a predicted MW of 35.78 kDa. Due to glycosylation, the protein migrates to 45-53 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-mFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 85% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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|
Immobilized Human DKK1 C terminal Domain, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA (QC Test). |
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Human DKK1 C terminal Domain on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human DKK1 C terminal Domain is greater than 85% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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