Human DKK1 Protein (DKK-HM201)
Catalog: DKK-HM201
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Product Description | Recombinant Human DKK1 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Thr32-His266. |
Product Background |
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. |
Product Category |
Recombinant Protein |
Target |
DKK1 |
Gene/Protein ID |
O94907 |
Species |
Human |
Exact Sequence |
Thr32-His266 |
Molecular Weight | The protein has a predicted MW of 52.54 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human DKK1, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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