Human DLL4 Protein (DLL-HM004)

  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser27-Pro524

Catalog: DLL-HM004

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Product Description Recombinant Human DLL4 Protein is expressed from HEK293 without tag.It contains Ser27-Pro524.

Product Background

Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.

Product Category

Recombinant Protein

Target

DLL4

Gene/Protein ID

Q9NR61

Species

Human

Exact Sequence

Ser27-Pro524
Molecular Weight The protein has a predicted MW of 54.28 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.
Human DLL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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