Human DLL4 Protein (DLL-HM004)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ser27-Pro524

Catalog: DLL-HM004

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Product Description	Recombinant Human DLL4 Protein is expressed from HEK293 without tag.It contains Ser27-Pro524.
Product Background	Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Product Category	Recombinant Protein

Target	DLL4
Accession	Q9NR61
Species	Human
Amino Acid Range	Ser27-Pro524
Molecular Weight	The protein has a predicted MW of 54.28 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human DLL4, No Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
	The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.
	Human DLL4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.