Human DLL4 Protein (DLL-HM104)
Human DLL4 Protein (DLL-HM104)
Human DLL4 Protein (DLL-HM104)
Human DLL4 Protein (DLL-HM104)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human DLL4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser27-Pro524
Catalog #DLL-HM104
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Product Description
 Recombinant Human DLL4 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ser27-Pro524.
Product Background
 Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Product Category
 Recombinant Protein
Target
 DLL4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser27-Pro524
Molecular Weight
 The protein has a predicted MW of 55.38 kDa. Due to glycosylation, the protein migrates to 56-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human DLL4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DLL4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DLL4 Antibody, hFc Tag with the EC50 of 1.6ng/ml determined by ELISA.
Human DLL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DLL4 is greater than 90% as determined by SEC-HPLC.
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