Name: Human DLL4 Protein (DLL-HM204)
Brand: KactusBio
SKU: DLL-HM204-100UG
Price: 223.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE.;> 95% as determined by HPLC
Activity: Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser27-Pro524

Catalog #DLL-HM204

Size

100UG

500UG

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Human DLL4 Protein (DLL-HM204) Summary

Product Description	Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Pro524.
Product Background	Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human DLL4 Protein (DLL-HM204) Specifications/Details

Protein	DLL4
Synonyms	DLL-4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4
Accession	Q9NR61
Species	Human
Biotinylated	no
Amino Acid Range	Ser27-Pro524
Molecular Weight	The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE.;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1 mg/ml for 500ug or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200).
	Immobilized Human Notch 1, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3 ng/ml determined by ELISA (QC Test).
	Immobilized Human DLL4, hFc Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5 ng/ml determined by ELISA.
	Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human DLL4 is greater than 95% as determined by SEC-HPLC.