Product Description |
Recombinant Human DLL4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ser27-Pro524. |
Product Background |
Delta-like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (By similarity). During spinal cord neurogenesis, inhibits V2a interneuron fate. |
Product Category |
Recombinant Protein |
Target |
DLL4 |
Synonyms |
DLL4; MGC126344; notch ligand DLL4; Delta4; Delta-like 4 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser27-Pro524 |
Molecular Weight |
The protein has a predicted MW of 81.1 kDa. Due to glycosylation, the protein migrates to 82-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DLL4, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.48 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human DLL4, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-DLL4 Antibody, hFc Tag with the EC50 of 23.5ng/ml determined by ELISA (QC Test). |
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Immobilized Human Notch 1, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 10.3ng/ml determined by ELISA. |
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Human DLL4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of- Human DLL4 is greater than 95% as determined by SEC-HPLC. |
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