Human DNAM-1/CD226 Protein (DAM-HM101)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
  • Activity: The affinity constant of 0.22μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu19-Asn247

Catalog: DAM-HM101

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Product Description Recombinant Human DNAM-1/CD226 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Glu19-Asn247.
Product Category Recombinant Protein
Target DNAM-1/CD226
Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Gene/Protein ID Q15762
Species Human
Exact Sequence Glu19-Asn247
Molecular Weight The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.22μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22μM as determined in a SPR assay (Biacore T200).
The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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