Product Description |
Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247. |
Product Background |
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
Product Category |
Recombinant Protein |
Target |
DNAM-1/CD226 |
Synonyms |
CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu19-Asn247 |
Molecular Weight |
The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
|
|
Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC. |
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