Name: Human DNAM-1/CD226 Protein (DAM-HM201)
Brand: KactusBio
SKU: DAM-HM201-100UG
Price: 223.3 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu19-Asn247

Catalog #DAM-HM201

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247.
Product Background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	DNAM-1/CD226
Synonyms	CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Accession	Q15762
Species	Human
Biotinylated	no
Amino Acid Range	Glu19-Asn247
Molecular Weight	The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.234 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human CD155, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).
	Human DNAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.