Human DNAM-1/CD226 Protein (DAM-HM201)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Glu19-Asn247

Catalog: DAM-HM201

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Product Description Recombinant Human DNAM-1/CD226 Protein is expressed from Expi293 with hFc tag at the C-terminal.It contains Glu19-Asn247.
Product Category Recombinant Protein
Target DNAM-1/CD226
Synonyms CD226 molecule; CD226; DNAM1; PTA1; TLiSA1
Gene/Protein ID Q15762
Species Human
Exact Sequence Glu19-Asn247
Molecular Weight The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 μM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA.
The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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