• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg (QC Test).
  • Amino Acid Range: Asn29-Pro766
Catalog #DPV-HM126
Price
Regular price $429.00
Regular price Sale price $429.00
/
Size
Avantor VWR Fisher Scientific

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Human DPPIV/CD26 Protein (DPV-HM126) Summary

Product Description

 Recombinant Human DPPIV/CD26 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn29-Pro766.

Product Background

 CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.

Human DPPIV/CD26 Protein (DPV-HM126) Specifications/Details

Protein
 DPPIV/CD26
Synonyms
 ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5;EC3.4.14.5
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asn29-Pro766
Molecular Weight
 The protein has a predicted MW of 86.39 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).. See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).. Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test)..
Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human DPPIV on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human DPPIV is greater than 95% as determined by SEC-HPLC. The purity of Human DPPIV is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...