Human DPPIV/CD26 Protein (DPV-HM226)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human DPPIV, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Asn29-Pro766

Catalog: DPV-HM226

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Product Description Recombinant Human DPPIV/CD26 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn29-Pro766.

Product Background

CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.

Product Category

Recombinant Protein

Target

DPPIV/CD26
Synonyms ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5

Gene/Protein ID

P27487

Species

Human

Exact Sequence

Asn29-Pro766
Molecular Weight The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human DPPIV, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human DPPIV, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA (QC Test).
Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 3500 pmol/min/µg (QC Test).
The purity of Human DPPIV is greater than 95% as determined by SEC-HPLC.
Human DPPIV on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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