- Express System: HEK293
- Product Tag: N-hFc (IgG1)
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human DPPIV, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 17.9 ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 3500 pmol/min/µg (QC Test).
- Amino Acid Range: Asn29-Pro766
Human DPPIV/CD26 Protein (DPV-HM226) Summary
Product Description |
Recombinant Human DPPIV/CD26 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Asn29-Pro766. |
Product Background |
CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human DPPIV/CD26 Protein (DPV-HM226) Specifications/Details
Protein |
DPPIV/CD26 |
Synonyms |
ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn29-Pro766 |
Molecular Weight |
The protein has a predicted MW of 111.2 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DPPIV, hFc Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 17.9 ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is > 3500 pmol/min/µg (QC Test). |
Product Tag |
N-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 20% Glycerol (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
Other Available Proteins:
Finding available proteins...
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