• Express System: HEK293
  • Product Tag: C-His-monomeric Fc
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Gln25-Gln199
Catalog #DR3-HM603
Price
Regular price $297.00
Regular price Sale price $297.00
/
Size
Avantor VWR Fisher Scientific

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Human DR3/TNFRSF25 Protein (DR3-HM603) Summary

Product Description

 Recombinant Human DR3/TNFRSF25 Protein is expressed from HEK293 with His tag and monomeric Fc tag at the C-terminus.It contains Gln25-Gln199.

Product Background

 Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human DR3/TNFRSF25 Protein (DR3-HM603) Specifications/Details

Protein
 DR3/TNFRSF25
Synonyms
 APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln25-Gln199
Molecular Weight
 The protein has a predicted MW of 49.80 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-monomeric Fc

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Loaded Human DR3, monomeric Fc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator). Loaded Human DR3, monomeric Fc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator).
Other Available Proteins:
Finding available proteins...
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