Human DR3/TNFRSF25 Protein (DR3-HM603)
Human DR3/TNFRSF25 Protein (DR3-HM603)
  • Express System: HEK293
  • Product Tag: C-His-hFc
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.67ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln25-Gln199
Catalog #DR3-HM603
Regular price $270.00
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Product Description
Recombinant Human DR3/TNFRSF25 Protein is expressed from HEK293 with His tag and hFc tag at the C-terminus.It contains Gln25-Gln199.
Product Background
Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.
Product Category
Recombinant Protein
Target
DR3/TNFRSF25
Synonyms
APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln25-Gln199
Molecular Weight
The protein has a predicted MW of 49.80 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.67ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-hFc
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 0.67ug/ml determined by ELISA (QC Test).
Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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