Human DR3/TNFRSF25 Protein (DR3-HM603)

Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).

Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).

Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Loaded Human DR3, monomeric Fc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator).

Express System: HEK293
Product Tag: C-His-monomeric Fc
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln25-Gln199

Catalog #DR3-HM603

Price

$297⁰⁰

Size

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Human DR3/TNFRSF25 Protein (DR3-HM603) Summary

Product Description

Recombinant Human DR3/TNFRSF25 Protein is expressed from HEK293 with His tag and monomeric Fc tag at the C-terminus.It contains Gln25-Gln199.

Product Background

Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.

Human DR3/TNFRSF25 Protein (DR3-HM603) Specifications/Details

Protein	DR3/TNFRSF25
Synonyms	APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Accession	AAI17190
Species	Human
Biotinylated	no
Amino Acid Range	Gln25-Gln199
Molecular Weight	The protein has a predicted MW of 49.80 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test). The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His-monomeric Fc
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human DR3, monomeric Fc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, monomeric Fc Tag with the EC50 of 2.2ug/ml determined by ELISA (QC Test).
	Human DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	Loaded Human DR3, monomeric Fc Tag on ProA-Biosensor, can bind Human TNFSF15, His Tag with an affinity constant of 5.04 nM as determined in BLI assay (Gator).