Product Description |
Recombinant Human E-Cadherin/Cadherin-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp155-Ala709. |
Product Background |
E-cadherin is the core component of epithelial adherens junctions, essential for tissue development, differentiation, and maintenance. It is also fundamental for tissue barrier formation, a critical function of epithelial tissues. |
Product Category |
Recombinant Protein |
Target |
E-Cadherin/Cadherin-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp155-Ala709 |
Molecular Weight |
The protein has a predicted MW of 61.62 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human E-Cadherin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human E-Cadherin is greater than 90% as determined by SEC-HPLC. |
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