Human E-Cadherin/Cadherin-1 Protein (CDH-HM101)
Catalog: CDH-HM101
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| Product Description | Recombinant Human E-Cadherin/Cadherin-1 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Asp155-Ala709. |
Product Background |
E-cadherin is the core component of epithelial adherens junctions, essential for tissue development, differentiation, and maintenance. It is also fundamental for tissue barrier formation, a critical function of epithelial tissues. |
Product Category |
Recombinant Protein |
Target |
E-Cadherin/Cadherin-1 |
Gene/Protein ID |
P12830-1 |
Species |
Human |
Exact Sequence |
Asp155-Ala709 |
| Molecular Weight | The protein has a predicted MW of 61.62 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCL (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human E-Cadherin is greater than 90% as determined by SEC-HPLC. |
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Human E-Cadherin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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