• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Trp22-Pro556
Catalog #ESE-HM201
Price
Regular price $19800
Regular price Sale price $19800
/
Size
Avantor VWR Fisher Scientific

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Human E-Selectin/CD62E Protein (ESE-HM201) Summary

Product Description

 Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Trp22-Pro556.

Product Background

 E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Human E-Selectin/CD62E Protein (ESE-HM201) Specifications/Details

Protein
 E-Selectin/CD62E
Synonyms
 CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Trp22-Pro556
Molecular Weight
 The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human E-selectin is greater than 90% as determined by SEC-HPLC. The purity of Human E-selectin is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...