Human E-Selectin/CD62E Protein (ESE-HM201)
Human E-Selectin/CD62E Protein (ESE-HM201)
Human E-Selectin/CD62E Protein (ESE-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp22-Pro556
Catalog #ESE-HM201
Regular price $180.00
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Product Description

Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp22-Pro556.

Product Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Product Category

Recombinant Protein / Antibody discovery

Protein

E-Selectin/CD62E

Synonyms

CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Trp22-Pro556

Molecular Weight

The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test).
Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC. The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.

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