Name: Human E-Selectin/CD62E Protein (ESE-HM201)
Brand: KactusBio
SKU: ESE-HM201-100UG
Price: 180.0 USD
Availability: InStock

Human E-Selectin/CD62E Protein (ESE-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Trp22-Pro556

Catalog #ESE-HM201

Size

100UG

500UG

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Product Description	Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp22-Pro556.
Product Background	E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Product Category	Recombinant Protein

Target	E-Selectin/CD62E
Synonyms	CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Accession	P16581
Species	Human
Biotinylated	no
Amino Acid Range	Trp22-Pro556
Molecular Weight	The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA.
	Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.

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