Human E-Selectin/CD62E Protein (ESE-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp22-Pro556

Catalog: ESE-HM201

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Product Description Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Trp22-Pro556.

Product Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Product Category

Recombinant Protein

Target

E-Selectin/CD62E
Synonyms CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2

Gene/Protein ID

P16581

Species

Human

Exact Sequence

Trp22-Pro556
Molecular Weight The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human E-Selectin, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 20.5ng/ml determined by ELISA.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
Human E-selectin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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