Human E-Selectin/CD62E Protein (ESE-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Trp22-Pro556

Catalog: ESE-HM401

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Product Description Recombinant Human E-Selectin/CD62E Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Trp22-Pro556.
Product Category Recombinant Protein
Target E-Selectin/CD62E
Synonyms CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Gene/Protein ID P16581
Species Human
Exact Sequence Trp22-Pro556
Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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