Human E-Selectin/CD62E Protein (ESE-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human E-Selectin, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Trp22-Pro556

Catalog: ESE-HM401

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Product Description	Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.
Product Background	E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Product Category	Recombinant Protein

Target	E-Selectin/CD62E
Synonyms	CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Gene/Protein ID	P16581
Species	Human
Exact Sequence	Trp22-Pro556
Molecular Weight	The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human E-Selectin, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human E-Selectin, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA.
	The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
	Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.