Human E-Selectin/CD62E Protein (ESE-HM401)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human E-Selectin, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Trp22-Pro556

Catalog: ESE-HM401

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Product Description Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Trp22-Pro556.

Product Background

E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.

Product Category

Recombinant Protein

Target

E-Selectin/CD62E
Synonyms CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2
Accession

Species

Human

Amino Acid Range

Trp22-Pro556
Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human E-Selectin, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human E-Selectin, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.
The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.
Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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