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Human E-Selectin/CD62E Protein, Ultra Low Endotoxin (ESE-HM201-UL) Summary
Product Description |
Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Trp22-Pro556. |
Product Background |
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human E-Selectin/CD62E Protein, Ultra Low Endotoxin (ESE-HM201-UL) Specifications/Details
Protein |
E-Selectin/CD62E |
Synonyms |
CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Trp22-Pro556 |
Molecular Weight |
The protein has a predicted MW of 85.4 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human E-Selectin, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-E-Selectin Antibody, hFc Tag with the EC50 31.4ng/ml determined by ELISA (QC Test). |
|
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Human E-selectin on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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