Human EFEMP1 Protein (EFE-HM101)
Human EFEMP1 Protein (EFE-HM101)
Human EFEMP1 Protein (EFE-HM101)
Human EFEMP1 Protein (EFE-HM101)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Gln18-Phe493
Catalog #EFE-HM101
Regular price $346.50
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Human EFEMP1 Protein (EFE-HM101) Summary

Product Description

Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln18-Phe493.

Product Background

Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human EFEMP1 Protein (EFE-HM101) Specifications/Details

Protein
EFEMP1
Synonyms
EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln18-Phe493
Molecular Weight
The protein has a predicted MW of 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA (QC Test).
Product Tag
N-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA (QC Test). Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA (QC Test).
Human EFEMP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EFEMP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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