Human EFEMP1 Protein (EFE-HM101)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln18-Phe493

Catalog: EFE-HM101

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Product Description Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln18-Phe493.

Product Background

Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.

Product Category

Recombinant Protein

Target

EFEMP1
Synonyms EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5

Gene/Protein ID

Q12805-1

Species

Human

Exact Sequence

Gln18-Phe493
Molecular Weight The protein has a predicted MW of 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 20.0ng/ml determined by ELISA.
The purity of Human EFEMP1 is greater than 95% as determined by SEC-HPLC.
Human EFEMP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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