Product Description |
Recombinant Human EFEMP1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln18-Phe493. |
Product Background |
Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
EFEMP1 |
Synonyms |
EFEMP1; FBLN3; FBNL; DHRD; FBNLFLJ35535; FIBL-3; fibrillin-like; Fibulin 3; MGC111353; MLVT; MTLV; S1-5 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln18-Phe493 |
Molecular Weight |
The protein has a predicted MW of 53.9 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EFEMP1, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 29.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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