Product Description |
Recombinant Human/Cynomolgus Ephrin-A3/EFNA3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln23-Ser213. |
Product Background |
Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development. |
Product Category |
Recombinant Protein |
Target |
EFNA3/Ephrin A3 |
Synonyms |
Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3 |
Accession |
|
Species |
Human/Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gln23-Ser213 |
Molecular Weight |
The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
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Human/Cynomolgus Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human/Cynomolgus Ephrin-A3 is greater than 95% as determined by SEC-HPLC. |
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