Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein (EFN-HM2A3)
Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein (EFN-HM2A3)
Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein (EFN-HM2A3)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln23-Ser213
Catalog #EFN-HM2A3
Regular price $120.00
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Product Description

Recombinant Human/Cynomolgus/Rhesus macaque Ephrin-A3/EFNA3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln23-Ser213.

Product Background

Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.

Product Category

Recombinant Protein / Antibody discovery

Protein

Ephrin-A3/EFNA3

Synonyms

Ephrin-A3; Ephrin A3; EFL-2; EHK1 ligand; EHK1-L; EFNA3; EPLG3; LERK3

Accession

Species

Human/Cynomolgus/Rhesus macaque

Biotinylated

no

Amino Acid Range

Gln23-Ser213

Molecular Weight

The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). Immobilized Human EPHA10, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human/Cynomolgus/Rhesus macaque Ephrin-A3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test).
Human/Cynomolgus/Rhesus macaque Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human/Cynomolgus/Rhesus macaque Ephrin-A3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human/Cynomolgus/Rhesus macaque Ephrin-A3 is greater than 95% as determined by SEC-HPLC. The purity of Human/Cynomolgus/Rhesus macaque Ephrin-A3 is greater than 95% as determined by SEC-HPLC.

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