Human EGF Protein (EGF-HM202)
Human EGF Protein (EGF-HM202)
Human EGF Protein (EGF-HM202)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Asn971-Arg1023
Catalog #EGF-HM202
Regular price $297.00
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Human EGF Protein (EGF-HM202) Summary

Product Description

Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn971-Arg1023.

Product Background

The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human EGF Protein (EGF-HM202) Specifications/Details

Protein
EGF
Synonyms
HOMG4; EGF; URG
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asn971-Arg1023
Molecular Weight
The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50-for this effect is typically 0.2 - 1.5 ng/mL. Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50-for this effect is typically 0.2 - 1.5 ng/mL.
The purity of Human EGF is greater than 95% as determined by SEC-HPLC. The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
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