Human EGF Protein (EGF-HM202)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Asn971-Arg1023

Catalog: EGF-HM202

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Product Description	Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn971-Arg1023.
Product Category	Recombinant Protein

Target	EGF
Gene/Protein ID	P01133-1
Species	Human
Exact Sequence	Asn971-Arg1023
Molecular Weight	The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).
	The purity of Human EGF is greater than 95% as determined by SEC-HPLC.
	Human EGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.