Name: Human EGF Protein (EGF-HM202)
Brand: KactusBio
SKU: EGF-HM202-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asn971-Arg1023

Catalog #EGF-HM202

Size

100UG

500UG

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Human EGF Protein (EGF-HM202) Summary

Product Description	Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn971-Arg1023.
Product Background	The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human EGF Protein (EGF-HM202) Specifications/Details

Protein	EGF
Synonyms	HOMG4; EGF; URG
Accession	P01133-1
Species	Human
Biotinylated	no
Amino Acid Range	Asn971-Arg1023
Molecular Weight	The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test). The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 4.1ng/ml determined by ELISA (QC Test).
	Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50-for this effect is typically 0.2 - 1.5 ng/mL.
	The purity of Human EGF is greater than 95% as determined by SEC-HPLC.