Product Description |
Recombinant Human EGF Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Asn971-Arg1023. |
Product Background |
The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family. |
Product Category |
Recombinant Protein |
Target |
EGF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn971-Arg1023 |
Molecular Weight |
The protein has a predicted MW of 32.52 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human EGF, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). |
|
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Immobilized Biotinylated Human EGFR, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate(5ug/ml). Dose response curve for Human EGF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test). |
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|
Human EGF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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|
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50-for this effect is typically 0.2 - 1.5 ng/mL. |
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The purity of Human EGF is greater than 95% as determined by SEC-HPLC. |
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